Template-Type: ReDIF-Article 1.0
Author-Name:Abdul Nafeh, Nayab Zahid, Sara Aleem, Hamza Shahab, Dr. Imran Afzal
Author-Email:nayab.z.gillani@gmail.com
Author-Workplace-Name:Department of Biology, Lahore Garrison University, Lahore, Department of Surgery, Sialkot Medical College, Sialkot, Department of Medicine, Sialkot Medical College, Sialkot, Department of Medicine, Islam Medical College, Sialkot
Title:Silver Nanoparticles Synthesis by Serratia Marcescens W2 Strain, Its Biocontrol Efficacy Against Fungal Phytopathogens, And Its Effect on Wheat Seeds
Abstract:Due   to   its   cost-effectiveness   and   eco-friendliness,   the   production   of   silver nanoparticles (AgNPs) utilizing biological agents has attracted a lot of attention. In this study, we investigate the potential of the Serratia marcescens W2 strain as a bio-factory for the production of silver nanoparticles and evaluate the biocontrol capability of this strain  against  fungal  phytopathogens  as  well  as  its  impact  on  wheat  seeds.  Using  the extracellular  enzymes  and  metabolites  produced  by  Serratia  marcescens  W2,AgNPswere biosynthesised.  The  size,  form,  and  composition  of  the  AgNPs  were  determined  using  a variety  of  analytical  techniques,  such  as  X-ray  diffraction  (XRD),  and  scanning  electron microscopy (SEM). AgNPs' impressive ability to inhibit fungal growth in vitro experiments demonstrates their robust biocontrol capabilities. Microscopic and biochemical investigations helped to better clarify the AgNPs' mode of action against these phytopathogens. Additionally, investigations on seed germination and seedling growth were used to evaluate the effect of the AgNPs on wheat seeds. As a result of the application of AgNPs, seed germination rates, and seedling vigor dramatically increased, according to the findings. Additionally, compared to the control  group,  the  seedlings  treated  with  AgNPs  showed  enhanced  resistance  to  fungal infection. Overall, the results of this study demonstrate the potential of Serratia marcescens W2 strain in the green synthesis of AgNPs with improved antifungal activities. Furthermore, the use of these AgNPs promotes the germination and growth of wheat seedlings, indicating their potential use as a biocontrol agent and seed treatment to safeguard crops against fungus phytopathogens   in   sustainable   agriculture.   To   completely   understand   the   underlyingmechanisms  and  determine  the  long-term  impacts  of  AgNPs  on  the ecosystem  and  human health, more research is necessary.
Keywords:Green technology, Microbial Synthesis, silver nanoparticles, intracellular synthesis
Journal:International Journal of Innovations in Science and Technology
Pages:871-880
Volume:7
Issue:2
Year:2025
Month:May
File-URL:https://journal.50sea.com/index.php/IJIST/article/view/1260/1898
File-Format: Application/pdf
File-URL:https://journal.50sea.com/index.php/IJIST/article/view/1260
File-Format: text/html
Handle: RePEc:abq:IJIST:v:7:y:2025:i:2:p:871-880